Journal: Frontiers in Oncology

Article Title: Scutellaria barbata Inhibits Hepatocellular Carcinoma Tumorigenicity by Inducing Ferroptosis of Hepatocellular Carcinoma Cells

doi: 10.3389/fonc.2022.693395

Figure Lengend Snippet: Primers used in this study.

Article Snippet: The primary antibodies used in the present study were diluted into 5% nonfat milk as follow: GPX4 antibody (PAC994Hu01, USCN, Wuhan, China, 1:1,000), ACSL4 antibody (A04372-2, Boster, Wuhan, China, 1:1,000), CCRB1 (SLC7A11) antibody (bs-6883R, Bioss, Beijing, China, 1:1,000), IREB2 antibody (PAH789Hu01, USCN, 1:1,000), and GAPDH antibody (KC-5G5, Aksomics, Shanghai, China, 1:10,000).

Techniques: Sequencing

Journal: Frontiers in Oncology

Article Title: Scutellaria barbata Inhibits Hepatocellular Carcinoma Tumorigenicity by Inducing Ferroptosis of Hepatocellular Carcinoma Cells

doi: 10.3389/fonc.2022.693395

Figure Lengend Snippet: Scutellaria barbata induces ferroptosis through regulating genes involved in lipid ROS metabolism and iron perioxidation in HCC cells. (A) The mRNA levels of GPX4, SLC7A11, IREB2, and ACSL4 were detected by RT-PCR in HCC cell lines (SMMC-7721, HepG2, and Huh7) with or without S. barbata treatment. (B) The protein levels of GPX4, SLC7A11, IREB2, and ACSL4 were detected by Western blot analysis in HCC cell lines (SMMC-7721, HepG2, and Huh7) with or without S. barbata treatment. Data were representative of three independent experiments and analyzed by unpaired t-test. Error bars denote SD. *p < 0.05.

Article Snippet: The primary antibodies used in the present study were diluted into 5% nonfat milk as follow: GPX4 antibody (PAC994Hu01, USCN, Wuhan, China, 1:1,000), ACSL4 antibody (A04372-2, Boster, Wuhan, China, 1:1,000), CCRB1 (SLC7A11) antibody (bs-6883R, Bioss, Beijing, China, 1:1,000), IREB2 antibody (PAH789Hu01, USCN, 1:1,000), and GAPDH antibody (KC-5G5, Aksomics, Shanghai, China, 1:10,000).

Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: Aging (Albany NY)

Article Title: Copper metabolism-related risk score identifies hepatocellular carcinoma subtypes and SLC27A5 as a potential regulator of cuproptosis

doi: 10.18632/aging.205334

Figure Lengend Snippet: The expression and function of SLC27A5 in HCC. ( A – C ) The transcriptional expression of SLC27A5 in HCC and normal tissues of TCGA-LIHC ( A ), GSE14520 ( B ) and GSE64041 ( C ) datasets. ( D ) The protein level of SLC27A5 in HCC and normal tissues. ( E , F ) CCK-8 assay for HepG2 ( E ) and LM-3 ( F ) cells overexpressing SL27A5. ( G , H ) HepG2 ( G ) and LM-3 ( H ) cells were subject to flow cytometry analysis for cell cycle. ( I ) HCC cells overexpressing SLC27A5 were subject to EdU staining. ( J ) HCC cells overexpressing SLC27A5 were subject to wound healing assay.

Article Snippet: Western blotting was performed using antibodies against FDX1 (M05441, Boster), SLC27A5 (A09287-2, Boster), GAPDH (BM3876, Boster).

Techniques: Expressing, CCK-8 Assay, Flow Cytometry, Staining, Wound Healing Assay

Journal: Aging (Albany NY)

Article Title: Copper metabolism-related risk score identifies hepatocellular carcinoma subtypes and SLC27A5 as a potential regulator of cuproptosis

doi: 10.18632/aging.205334

Figure Lengend Snippet: SLC27A5 upregulates FDX1 in HCC. ( A , B ) The correlation between the expression of SLC27A5 and that of FDX1 in TCGA-LIHC ( A ) and GSE14520 ( B ) datasets. ( C ) Dot plot showing the dependency scores for SLC27A5 and FDX1 across all tumor cell lines in the Project Achilles/Cancer Dependency Map Portal (DepMap). ( D , E ) The expression of and correlation between SLC27A5 and FDX1 in collected HCC tissue chip. ( F , G ) HepG2 ( F ) and LM-3 ( G ) cells overexpressing SLC27A5 were subject to qRT-PCR analysis. ( H , I ) Western blotting using lysates of HepG2 ( H ) and LM-3 ( I ) cells after overexpressing SLC27A5.

Article Snippet: Western blotting was performed using antibodies against FDX1 (M05441, Boster), SLC27A5 (A09287-2, Boster), GAPDH (BM3876, Boster).

Techniques: Expressing, Quantitative RT-PCR, Western Blot